Efficacy of amelogenin-chitosan hydrogel in biomimetic repair of human enamel in pH-cycling systems

Qichao Ruan, David Liberman, Rucha Bapat, Karthik Balakrishna Chandrababu, Jin-Ho Phark, Janet Moradian-Oldak


Amelogenin-chitosan (CS-AMEL) hydrogel has shown great potential for the prevention, restoration, and treatment of defective enamel. As a step prior to clinical trials, this study aimed to examine the efficacy of CS-AMEL hydrogel in biomimetic repair of human enamel with erosive or caries-like lesions in pH-cycling systems. Two models for enamel defects, erosion and early caries, were addressed in this study. Two pH-cycling systems were designed to simulate the daily cariogenic challenge as well as the nocturnal pH conditions in the oral cavity. After pH cycling and treatment with CS-AMEL hydrogel, a synthetic layer composed of oriented apatite crystals was formed on the eroded enamel surface. CS-AMEL repaired the artificial incipient caries by re-growing oriented crystals and reducing the depth of the lesions by up to 70% in the pH-cycling systems. The results clearly demonstrate that the CS-AMEL hydrogel is effective at the restoration of erosive and carious lesions under pH-cycling conditions.


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DOI: https://doi.org/10.5430/jbei.v2n1p119


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Journal of Biomedical Engineering and Informatics

ISSN 2377-9381(Print)  ISSN 2377-939X(Online)

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