The application of sequence specific primer and RT-PCR to LRRK2 gene polymorphism typing

Biao G, Gaisheng T, Qinxue L, Fengrui L


Objective: To establish a new detecting method for disease susceptibility loci R1628P and G2385R of Parkinson’s disease (PD) related gene LRRK2.

Methods: Sequence specific primers were designed to make a genotyping of DNA markers with known genotypes by use of quantitative fluorescence real-time PCR (RT-PCR). 100 cases of PD samples with unknown genotypes were tested, and verified by use of polymerase chain reaction linked restriction fragment length polymorphism (PCR-RLFP).

Results: The genotyping results of DNA markers proved to be correct, and 100 cases of samples to be tested had a completely consistent genotyping result with PCR-RLFP genotyping result.

Conclusions: Sequence specific primer and quantitative fluorescence RT-PCR can successfully make a genotyping for disease susceptibility loci R1628P and G2385R of LRRK2.

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Discussion of Clinical Cases  ISSN 2375-8449(Print)  ISSN 2375-8473(Online)

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